Faculty of Science

 

Please have some idea of how much sample is in your tube and use the following as a rough guide for pure compounds (of molecular weight 600 g/mol or less) on the AVANCE 400.
 

  50 mg or more 20 mg -50 mg 10 mg -20 mg 5 mg - 10 mg Less than 5 mg
Proton 16 scans 16 scans 16 scans 16  -32 scans  32 - 128 scans
Carbon 5 minutes 20 minutes 1 - 2 hours 2 hours >1 hours (use other spectromeyter)
COSY 5 minutes 5 minutes 10 minutes 20 minutes or more 1 hour or more
HMQC / HSQC 10 minutes 10 minutes 20 minutes 20 minutes or more 1 hour or more
HMBC 20 minutes 20 minutes 1 hour 1 hour >1 hours (use other spectromeyter)
NOESY 1 hour 1  hour 1 hour 1 hour >1 hours (use other spectromeyter)
Phosphorus 1 minute 1 minute 1  - 5 minutes 1  - 10 minutes 1  - 20 minutes
Fluorine 1 minute 1 minute 1 minute 1 - 5  minutes 1 - 10  minute